Membrane chloride conductance and capacitance in Jurkat T lymphocytes during osmotic swelling
نویسندگان
چکیده
منابع مشابه
Chloride channels activated by osmotic stress in T lymphocytes
We have used whole-cell and perforated-patch recording techniques to characterize volume-sensitive Cl- channels in T and B lymphocytes. Positive transmembrane osmotic pressure (intracellular osmolality > extracellular osmolality) triggers the slow induction of a Cl- conductance. Membrane stretch caused by cellular swelling may underlie the activation mechanism, as moderate suction applied to th...
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Osmotic cell swelling activates C l 2 channels to achieve anion efflux. In this study, we find that both the tyrosine kinase inhibitor herbimycin A and genetic knockout of p56 lck , a src -like tyrosine kinase, block regulatory volume decrease (RVD) in a human T cell line. Activation of a swelling-activated chloride current (I C l 2 swell ) by osmotic swelling in whole-cell patch-clamp experime...
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1 Recently, we provided evidence for cyclic adenosine 5'-diphosphate-ribose, cADP-ribose, as a second messenger in Jurkat T-lymphocytes upon stimulation of the T-cell receptor/CD3- complex (Guse et al., 1999). cADP-ribose mobilizes Ca2+ from an intracellular Ca2+ store which is sensitive to caffeine and gated by the ryanodine receptor/Ca2+ release channel. In the present study we investigated t...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1994
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(94)80754-4